A specific tripeptidyl substrate for tripeptidyl peptidase activity is effectively hydrolyzed by alanyl aminopeptidase/aminopeptidase N/CD13 in the rat kidney

A specific tripeptidyl substrate for tripeptidyl peptidase activity is effectively hydrolyzed by alanyl aminopeptidase/aminopeptidase N/CD13 in the rat kidney

M Shibata, M Koike, S Kusumi, N Sato… - Archives of Histology …, 2016 - jstage.jst.go.jp

... et al., 2002). Briefly, the activities were quantified in the reaction buffer (50 mM sodium
acetate, 1 mM EDTA, inhibitor cocktail (Roche), pH 5.5), containing 20 μM substrates
(Ala-Ala-Phe-MCA; Peptide Institute Inc. Osaka) that ...

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