Cytoplasmic kinases downstream of GPR30 suppress gonadotropin-releasing hormone (GnRH)-induced luteinizing hormone secretion from bovine anterior pituitary …

Cytoplasmic kinases downstream of GPR30 suppress gonadotropin-releasing hormone (GnRH)-induced luteinizing hormone secretion from bovine anterior pituitary …

FO Rudolf, H Kadokawa - Journal of Reproduction and Development, 2016 - jstage.jst.go.jp

... Cells were incubated with gentle shaking for 5 min, after which they were incubated for 2 h with
5 µl of 100 nM GnRH (Peptide Institute, Osaka, Japan) to stimulate LH secretion. Fig. 1 summarizes
the final concentrations of H89, U0126 and GnRH for each treatment. ...

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com