Direct mapping of additional modifications on phosphorylated O-glycans of α-dystroglycan by mass spectrometry analysis in conjunction with knocking out of causative …
H Yagi, CW Kuo, T Obayashi, S Ninagawa… - Molecular & Cellular …, 2016 - ASBMB
... At 24 h after transfection, cells were lysed in buffer A (50 mm Tris/Cl, pH 8.0, containing 1%
Nonidet P-40, 150 mm NaCl, protease inhibitor mixture (Nacalai Tesque, Kyoto, Japan), 20
μm MG132 (Peptide Institute, Osaka, Japan), and 2 μm Z-VAD-fmk (Promega, Madison, WI)). ...
Nonidet P-40, 150 mm NaCl, protease inhibitor mixture (Nacalai Tesque, Kyoto, Japan), 20
μm MG132 (Peptide Institute, Osaka, Japan), and 2 μm Z-VAD-fmk (Promega, Madison, WI)). ...
Eva He
eva@pepmic.com
Pepmic Co., Ltd
www.pepmic.com
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