Two‐step ion‐exchange chromatographic purification combined with reversed‐phase chromatography to isolate C‐peptide for mass spectrometric analysis

Two‐step ion‐exchange chromatographic purification combined with reversed‐phase chromatography to isolate C‐peptide for mass spectrometric analysis

K Kabytaev, A Durairaj, D Shin… - Journal of separation …, 2016 - Wiley Online Library

... The obtained fraction contained protein and peptides with pI values inside the pH
range of two and a half pH units. ... Louis, MO); isotopically labeled [D8]-Val7,10
C-peptide was obtained from Bachem (Bubendorf, Switzerland). ...

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