Site-saturation mutagenesis of mutant L-asparaginase II signal peptide hydrophobic region for improved excretion of cyclodextrin glucanotransferase
A Ismail, RM Illias - Journal of industrial microbiology & biotechnology, 2017 - Springer
Abstract The excretion of cyclodextrin glucanotransferase (CGTase) into the culture medium
offers significant advantages over cytoplasmic expression. However, the limitation of
Escherichia coli is its inability to excrete high amount of CGTase outside the cells. In this
offers significant advantages over cytoplasmic expression. However, the limitation of
Escherichia coli is its inability to excrete high amount of CGTase outside the cells. In this
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