Crude peptides are not recommended for biological assays. Crude peptides may contain large amounts of non-peptide impurities such as residual solvents, scavengers from cleavage, TFA and other truncated peptides. TFA cannot be totally removed. Peptides are usually delivered as TFA salt. If residual TFA is a problem for your experiment, we recommend other salt forms such as acetate and hydrochloride. These salt forms are usually 20-30% more expensive than the regular TFA salt. This is due to the peptide loss that takes place during the salt conversion and the greater amounts of raw materials required.
Pepmic recommends the following levels of peptide purity for various projects:
>70% purity
- Peptide arrays
- Antigens for antibody production
- Competitive elution chromatography
- ELISA standards for measuring antisera titers
>80% purity
- Western blotting studies (non-quantitative)
- Enzyme-substrate studies (non-quantitative)
- Peptide blocking studies (non-quantitative)
- Affinity purification
- Phosphorylation assays
- Protein electrophoresis applications and immunocytochemistry
>95% purity
- ELISA standards and RIA protocols (quantitative)
- Receptor-ligand interaction studies (quantitative)
- In vitro bioassays and in vivo studies
- Enzyme studies and blocking assays (quantitative)
- NMR studies
- Mass spectrometry
- Other quantitative assays
>98% purity
- SAR Studies
- Clinical trials
- APIs (Active Pharmaceutical Ingredients)
- Commercial products
- X-ray crystallography studies
- Other sensitive experiments: enzyme-substrate studies, receptor-ligand interaction studies, blocking and competition assays
For any question, please contact me at
Eva He
eva@pepmic.com
Pepmic Co., Ltd
www.pepmic.com
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