2017年3月22日星期三

Biotinylated Peptides


Biotinylation is a widely applied modification in life sciences research. The biotin moiety has a very strong affinity for streptavidin (Kd < 10-10 M) and biotinylation of peptides is therefore an efficient method to specifically bind peptides to streptavidin coated surfaces.

Biotinylation of peptides can be performed either at the N- or C-terminus. Biotinylation at the N-terminus can be performed directly to the primary-terminal amino group, whereas biotinylation is usually performed at the ε-amino group of an (extra) C-terminal lysine.

In case the distance between the biotin unit and the peptide is important, for instance to avoid sterical hindrance, a linker can be inserted between biotin and the peptide sequence. The 6-carbon ε-aminocaproic (Ahx) is a popular spacer; however, other spacers can also be applied based upon specific requirement.


Biotinylated Peptide



Eva He
eva@pepmic.com
Pepmic Co., Ltd.
www.pepmic.com

1 条评论:

  1. A variety of peptides are used in cosmetics. According to their mode of action, they have been classified into four main groups: signal peptides, Cosmetic Peptides

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