Peptide Synthesis: 四月 2017

2017年4月19日星期三

Peptidomic analysis of type 1 diabetes associated HLA‐DQ molecules and the impact of HLA‐DM on peptide repertoire editing

Z Zhou, E Reyes‐Vargas, H Escobar… - European Journal of …, 2017 - Wiley Online Library

... Peptides listed in Table 1 were commercially synthesized (PepMic). The N-terminus acetylated

CLIP (VSKMRMATPLLMQ) and HA (PRFVKQNTLRLAT) were labeled on the ϵ-amine of lysine (underlined)

residues with Alexa Fluor 488 5-TFP (Molecular Probes) as described ...

Peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

Architecture of the mitochondrial calcium uniporter

K Oxenoid, Y Dong, C Cao, T Cui, Y Sancak… - Nature, 2016 - nature.com

Approximately 0.4 mg synthesized peptide (Pepmic Co., Ltd) was dissolved in 1 ml of reaction buffer(25 mM sodium phosphate, pH 7.5, and 50 mM NaCl) to a concentration of 0.1 mM.

A stock solution of 30 mM DTSSP in water was prepared for addition to the peptide solution....

Peptide synthesis, peptide modification and peptide library synthesis library, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

[HTML] An intrinsic agonist mechanism for activation of glucagon-like peptide-1 receptor by its extracellular domain

Y Yin, XE Zhou, L Hou, LH Zhao, B Liu, G Wang… - Cell …, 2016 - ncbi.nlm.nih.gov

... Trypsin-EDTA and PBS were from Gibco. GLP-1(7–37), EX4(1–39) and GLP-1(9–37) for cAMP
and tango assays were custom-synthesized and high-performance liquid chromatography-purified
by Pepmic, and the EX4(3–39) peptides were purchased from Novopro. ...

Peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

[HTML] Structure of the homodimeric androgen receptor ligand-binding domain

M Nadal, S Prekovic, N Gallastegui… - Nature …, 2017 - ncbi.nlm.nih.gov

A peptide corresponding to residues S59-V72 of human UBA3 was custom-synthesized at Pepmic. Recombinant human AR-LBD (residues 662–919) was expressed as a fusion protein with thioredoxin and purified to homogeneity using standard purification methods23.

Peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eva He
eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

[HTML] Formation of bacterial pilus-like nanofibres by designed minimalistic self-assembling peptides

T Guterman, M Kornreich, A Stern… - Nature …, 2016 - nature.com

All peptides were synthesized by Pepmic Co., Ltd. (Suzhou, China), except for the N-terminal 4-mer peptide, the C-terminal 11-mer and the four C-terminal control peptides which......

Peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

The potential of the Galleria mellonella innate immune system is maximized by the co-presentation of diverse antimicrobial peptides

MR Bolouri Moghaddam, M Tonk, C Schreiber… - Biological …, 2016 - degruyter.com

...number: AAN15789; Seitz et al., 2003) as presented in Table 1. The peptides were produced

by Coring System Diagnostix(Gernsheim, Germany) and PepMic(Suzhou, China) using

solid-phase synthesis as previously described(Tonk, et al., 2015). Lyophilized peptides were...

Peptide synthesis, peptide midification and peptide library synthesis, please comntact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

Self-assembly and morphological characterization of two-component functional amyloid proteins

Q Qi, TX Zhao, BL An, XY Liu, C Zhong - Chinese Chemical Letters, 2016 - Elsevier

... were imaged through a Bio-Rad ChemiDoc MP system. 4.4. Sample preparation of
synthetic peptides. The loop5 peptide (R5) from CsgA monomer was synthesized
by Pepmic Corporation. 1 mg peptides were dissolved in 1 mL ...

Peptide synthesis, peptide modification and peptide library, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

Novel assay for early detection of a disease using a magnetic nanoparticle biosensor

M Zourob - US Patent App. 14/819,195, 2015 - Google Patents

... Company Inc. The peptide sequences (HNE specific peptide: GSGSGGGAAPV
AAKGGGSGSC and Cathepsin-G specific peptide: Ahx-GPQGIWGQR-Ahx) was
synthesised by Pepmic Co., Ltd (Suzhou, China). The coupling ...

Peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

添加图片说明

Biosensor using magnetic particles for pathogen detection

M Zourob - US Patent App. 14/867,353, 2015 - Google Patents

... The peptide sequence (Seq Id #1) NH 2 -Ahx-Gly-Gly-Gly-Ahx-Cys was synthesised by Pepmic
Co., Ltd. (Suzhou, China). ... The peptide sequence (Seq Id #2) NH 2 -Ahx-ETKVEENEAIQK-
Ahx-Cys was synthesised by Pepmic Co., Ltd. (Suzhou, China). ...

Peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

Identification and characterization of SMU. 244 encoding a putative undecaprenyl pyrophosphate phosphatase protein required for cell wall biosynthesis and …

N Jalal, XL Tian, G Dong, J Upham… - …, 2015 - mic.microbiologyresearch.org

... Both LcnG-a and LcnG-b were commercially synthesized with 90 % purity (Pepmic). Both peptides
were freshly dissolved at 100 mM in sterile distilled water, and then mixed at 1:1 molar ratio for
assembly before further dilutions as required for MIC and killing assays. ...

Peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

Phytaspase‐mediated precursor processing and maturation of the wound hormone

Phytaspase‐mediated precursor processing and maturation of the wound hormone systemin

RE Beloshistov, K Dreizler, RA Galiullina… - New …, 2017 - Wiley Online Library

... Cells were allowed to equilibrate at 120 rpm for at least 60 min before test peptides were added
from aqueous stock solutions in a volume of 50 ll. The systemin peptide and its derivatives were
obtained from PepMIC (Souzhou, China) at > 95% purity. Results ...

Peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

The subtilisin-like protease SBT3 contributes to insect resistance in tomato

M Meyer, F Huttenlocher, A Cedzich… - Journal of …, 2016 - Soc Experiment Biol

... after subculture (Schaller and Oecking, 1999). Synthetic systemin peptide (Pepmic;
Suzhou, China) was added from a 1000-fold concentrated stock solution in water.
Proteinase inhibitor assay. Four-week-old tomato plants were ...

Peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

Motif‐Designed Peptide Nanofibers Decorated with Graphene Quantum Dots for Simultaneous Targeting and Imaging of Tumor Cells

Z Su, H Shen, H Wang, J Wang, J Li… - Advanced Functional …, 2015 - Wiley Online Library

... applications. 4 Experimental Section. Reagents and Materials: Natural graphite rods
were purchased from Shanghai Tansu Factory (Shanghai, China). Peptides were
custom-ordered from Pepmic Co., Ltd. (95% purity, Suzhou, China). ...

Peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

Novel members of the adipokinetic hormone family in beetles of the superfamily Scarabaeoidea

G Gäde, P Šimek, HG Marco - Amino acids, 2016 - Springer

... 2010). Synthetic peptides. The novel adipokinetic peptides elucidated in this study,
Dorpa-AKH, Cirba-AKH, and Euoin-AKH (and in some cases, isomers too) were
purchased from Pepmic Co., Ltd. (Suzhou, China). Results. ...

Peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eve He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

Determination of noncovalent binding using a continuous stirred tank reactor as a flow injection device coupled to electrospray ionization mass spectrometry

IC Santos, VB Waybright, H Fan, S Ramirez… - Journal of The American …, 2015 - Springer

... Tripeptide stereoisomers of diacetyl-lysyl-alanyl-alanine (Ac-L-Lys(Ac)-L-Ala-L-Ala,
Ac-L-Lys(Ac)-D-Ala-L-Ala, Ac-L-Lys(Ac)-D-Ala-D-Ala, and Ac-L-Lys(Ac)-L-Ala-D-Ala) were
synthesized and purified to >95% purity (according to the manufacturer) by Pepmic Co., Ltd. ...

Peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

Two novel tyrosine-containing peptides (Tyr4) of the adipokinetic hormone family in beetles of the families Coccinellidae and Silphidae

G Gäde, P Šimek, HG Marco - Amino acids, 2015 - Springer

... 2010). Synthetic peptides. The novel adipokinetic peptides elucidated in this study,
Nicve-AKH from the CC of the burying beetle and Harax-AKH from the CC of the harlequin
ladybird beetle, were custom-synthesized by Pepmic Co., Ltd. ...

Peptide synthesis, peptide library synthesis and peptide modification, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

[HTML] Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus …

BP Lima, W Shi, R Lux - MicrobiologyOpen, 2017 - Wiley Online Library

... pVA8912 (Vickerman, Mansfield, Zhu, Walters, & Banas, 2015) into the wild-type S. gordonii V288
according to previously published protocol (Warren, Lund, Jones, & Hruby, 2007), utilizing
competence-stimulating peptide N-DVRSNKIRLWWENIFFNKK (Pepmic, Suzhou, China ..

Peptide synthesis, peptide library synthesis, peptide modification. Please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd.

www.pepmic.com

Novel Group of Leaderless Multipeptide Bacteriocins from Gram-Positive Bacteria

KV Ovchinnikov, H Chi, I Mehmeti, H Holo… - Applied and …, 2016 - Am Soc Microbiol

... Synthetic peptides.All the peptides were synthesized by Pepmic Co., Ltd., China, with 90 to
99% purity, except for CehA, CehB, CexA, and CevA (85% purity) due to technical difficulties
(poor purification). All the synthesized peptides were not formylated. ...

For peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

Molecular characterization, expression analysis, and bactericidal activity of the derivative peptides of TFPI-1 and TFPI-2 in half-smooth tongue sole, Cynoglossus …

X Zhao, S He, B Yue, G Wang, M Zhang - Fish & Shellfish Immunology, 2016 - Elsevier

... CsTFPI-1 corresponding to the C-terminal residues 254 to 277, TC38 (RNCMEVCVKGEKKH
TGQGMIRRLRRNKNNSIFVVRKRV) of CsTFPI-2 corresponding to the C-terminal residues 191
to 228, and the control peptide P86P15 [40] were synthesized by Pepmic (Suzhou, China ...

Peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

Identification and bioactivity evaluation of the first neuropeptide from the lesser-known insect order Embioptera (webspinner)

G Gäde, P Šimek, HG Marco - Amino acids, 2016 - Springer

... The novel AKH peptide of the webspinner O. saundersii, code-named Olisa-AKH,
and its analog N 6 P 7 -Olisa-AKH were commercially synthesized by Pepmic Co.,
Ltd. (Suzhou, China). Results. Presence of AKH peptide(s) in ...

Peptide synthesis, peptide modification, peptide library synthesis, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

Identification of a novel lipid binding motif in apolipoprotein B by the analysis of hydrophobic cluster domains

SM Gordon, M Pourmousa, M Sampson… - … et Biophysica Acta (BBA …, 2017 - Elsevier

... server [26]. 2.3. Synthesis of hydrophobic cluster domain containing peptides.
Peptides were prepared by solid phase synthesis (Pepmic Co.), purified to > 95%
by HPLC and confirmed by mass spectrometry. The sequences ...

Peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

Arsenic-induced stress activates sulfur metabolism in different organs of garlic (Allium sativum L.) plants accompanied by a general decline of the NADPH-generating …

C Ruíz-Torres, R Feriche-Linares… - Journal of Plant …, 2017 - Elsevier

... Phytochelatin 2 (PC2: H-γ- Glu- Cys-γ- Glu – Cys – Gly- OH) and phytochelatin 3
(PC3: H-γ- Glu-Cys-γ- Glu-Cys-γ-Glu-Cys- Gly-OH) were synthesized (Pepmic Co.,
Ltd, Suzhou, China) and used as standard for quantification. ...

Peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

Protective effect of Ac-SDKP on alveolar epithelial cells through inhibition of EMT via TGF-β1/ROCK1 pathway in silicosis in rat

H Deng, H Xu, X Zhang, Y Sun, R Wang… - Toxicology and applied …, 2016 - Elsevier

... β1 stimulation group: serum-free DMEM or MEM + 5 ng/ml TGF-β1; 3) an Ac-SDKP intervention
group: 80 mmol/L Ac-SDKP was added 1 h prior to 5 ng/ml TGF-β1 treatment; and 4) an Ac-ADKP
intervention group: 80 mmol/L Ac-ADKP (PCM10745-0912, PEPMIC, China) was ...

Peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eva He
eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

Structure–activity relationship of adipokinetic hormone analogs in the striped hawk moth, Hippotion eson

HG Marco, G Gäde - Peptides, 2015 - Elsevier

... detail previously [23]. Synthetic peptides. Peptides were custom-synthesized by Life
Tein (South Plainfield, NJ, USA; N-[acetyl]Glu 1 , Ile 2 , Val 2 , Asn 3 , Pro 6 and Ala
8 analogs of Lacol-AKH), Pepmic Co., Ltd. (Suzhou, China ...

Peptide synthesis, peptide library synthesis, please contact:

Eva He
eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

Proinsulin and MAP3865c homologous epitopes are a target of antibody response in new‐onset type 1 diabetes children from continental Italy

S Masala, D Cossu, S Piccinini, N Rapini… - Pediatric …, 2015 - Wiley Online Library

... and MAP 1,4-α-glucan branching protein (gbp) 157–173 [GTVELLGGPLAHPFQPL] along with
their respective homologous peptides PI 46–61 [RGFVVLPVTRRDVTDV], PI 64–80
[GQVELGGGPGAGSLQPL], reported in Table 1 were synthesized at >85% purity (Pepmic Co., Ltd...

Peptide synthesis, peptide modification and peptide library synthesis, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

Rapid and low-cost biosensor for the detection of Staphylococcus aureus

GARY Suaifan, S Alhogail, M Zourob - Biosensors and Bioelectronics, 2017 - Elsevier

... Self-adhesive Magnet sheets were purchased from Polarity Magnets Company (UK). The peptide
sequence NH 2 -Ahx-ETKVEENEAIQK-Ahx-Cys was synthesised by Pepmic Co., Ltd. (Suzhou,
China). Self-adhesive tape was purchased from Whatman (London, UK). ...

Peptide synthesis, Peptide modification, peptide library synthesis. Please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd

www.pepmic.com

[HTML] Antiplasmodial activity is an ancient and conserved feature of tick defensins

A Cabezas-Cruz, M Tonk, A Bouchut… - Frontiers in …, 2016 - ncbi.nlm.nih.gov

... Synthetic Peptides. The STiDA sequence information above was used to synthesize
the 55 amino acids mature peptides STiDA-1 and STiDA-2 using Fmoc solid phase
peptide synthesis (SPPS) with ∼ 95% purity (Pepmic, China). ...

For peptide synthesis, peptide modification, peptide library synthesis, please contact:

Eva He

eva@pepmic.com

Pepmic Co., Ltd.

www.pepmic.com

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