2017年5月31日星期三

Possible inhibitory roles of endogenous 2-arachidonoylglycerol during corticotropin-releasing factor-induced activation of central sympatho-adrenomedullary outflow …

Possible inhibitory roles of endogenous 2-arachidonoylglycerol during corticotropin-releasing factor-induced activation of central sympatho-adrenomedullary outflow …

T Shimizu, L Lu, K Yokotani - European journal of pharmacology, 2010 - Elsevier
... The following drugs were used: synthetic CRF (rat/human) (Peptide Institute, Osaka, Japan);
MAFP (methyl arachidonoyl fluorophosphonate), AM 251 [N-(piperidin-1-yl)-5-(4-iodophenyl)-
1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide], AM 404 [N-(4-hydroxyphenyl ...


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The three-finger toxin MTα is a selective α 2B-adrenoceptor antagonist

The three-finger toxin MTα is a selective α 2B-adrenoceptor antagonist

K Koivula, S Rondinelli, J Näsman - Toxicon, 2010 - Elsevier
... Fura-2 acetoxymethyl ester was from Molecular Probes (Eugene, OR). Venomous MTα purified
from D. polylepis was a kind gift from Dr. E. Karlsson (Uppsala, Sweden) and synthetic MTα was
from Peptide Institute (Osaka, Japan). 2.2. Cell culture and receptor expression. ...


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Engineered mutation of some important amino acids in angiotensin II type 1 (AT1) receptor increases the binding affinity of AT1-receptor antagonists

Engineered mutation of some important amino acids in angiotensin II type 1 (AT1) receptor increases the binding affinity of AT1-receptor antagonists

MA Bhuiyan, M Hossain, M Ishiguro… - Journal of …, 2010 - jstage.jst.go.jp
... software. Statistical analyses were performed by Stu- dent's t-test. Drugs Ang II used
in the study was purchased from Peptide Institute, Inc., Osaka. Candesartan was
obtained as gift from Takeda Chemical Industries Ltd., Osaka. ...


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Characterization of a legumain/vacuolar processing enzyme and YVADase activity in Papaver pollen

Characterization of a legumain/vacuolar processing enzyme and YVADase activity in Papaver pollen

M BoschNS Poulter, RM Perry, KA Wilkins… - Plant molecular …, 2010 - Springer
... substrates: Ac-DEVD-AMC, Ac-VEID-AMC, Ac-LEVD-AMC, Ac-YVAD-AMC, Ac-IETD-AMC,
Ac-LEHD-AMC (EMD Biosciences, San Diego, CA, and BioMol, Plymouth Meeting, PA) or the
4-methyl-coumaryl-7-amide (MCA) substrate Boc-GRR-MCA (Peptide Institute Inc., Osaka ...


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Mff is an essential factor for mitochondrial recruitment of Drp1 during mitochondrial fission in mammalian cells

Mff is an essential factor for mitochondrial recruitment of Drp1 during mitochondrial fission in mammalian cells

H Otera, C Wang, MM Cleland, K Setoguchi… - The Journal of cell …, 2010 - jcb.rupress.org


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Real‐time reaction monitoring by probe electrospray ionization mass spectrometry

Real‐time reaction monitoring by probe electrospray ionization mass spectrometry

Z YuLC Chen, R Erra‐Balsells… - Rapid …, 2010 - Wiley Online Library
... Louis, MO, USA). D 2 O (99.9 atom %D) was purchased from Wako Pure Chemicals (Osaka,
Japan). Angiotensin I (human) and bradykinin were obtained from Peptide Institute (Osaka,
Japan). Benzaldehyde was purchased from TCI (Tokyo, Japan). ...


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Controlled emission of platinum (II) terpyridyl complexes with poly-l-glutamic acid

Controlled emission of platinum (II) terpyridyl complexes with poly-l-glutamic acid

T Moriuchi, M Yamada, K Yoshii, T Hirao - Journal of Organometallic …, 2010 - Elsevier
... Poly-l-glutamic acid sodium salt with mol. wt. >8000 was obtained from PEPTIDE INSTITUTE,
INC. [Pt(trpy)Cl](OTf) [1e] and [Pt(trpy)C CPh](OTf) PtH[1u] were prepared by the literature
methods. All manipulations were carried out under argon. ...


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Vacuolar processing enzyme plays an essential role in the crystalline structure of glutelin in rice seed

Vacuolar processing enzyme plays an essential role in the crystalline structure of glutelin in rice seed

T Kumamaru, Y Uemura, Y Inoue… - Plant and Cell …, 2010 - academic.oup.com
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Serotonin Receptors are Involved in the Vagal Afferent Transmission of Exogenous Ghrelin-Evoked Appetite Sensation Mediated Though C-Fibers

Serotonin Receptors are Involved in the Vagal Afferent Transmission of Exogenous Ghrelin-Evoked Appetite Sensation Mediated Though C-Fibers

T Matsunaga - Acta Medica Nagasakiensia, 2010 - jstage.jst.go.jp
... In H&GVX, the hepatic branch plus bilateral gastric branches of the vagus nerves were resected
at 2 mm distal from their junction of the each trunk. Experimental protocol: Lyophilized rat ghrelin
(Peptide Institute. Inc., Osaka, Japan) was freshly dissolved in saline Page 3. ...


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Neprilysin Is Identical to Skin Fibroblast Elastase ITS ROLE IN SKIN AGING AND UV RESPONSES

Neprilysin Is Identical to Skin Fibroblast Elastase ITS ROLE IN SKIN AGING AND UV RESPONSES

N Morisaki, S Moriwaki, Y Sugiyama-Nakagiri… - Journal of Biological …, 2010 - ASBMB
... EXPERIMENTAL PROCEDURES. Materials. The synthetic substrate for elastase,
N-succinyl-tri-alanyl-p-nitroaniline (STANA), was purchased from the Peptide Institute Inc.
(Osaka, Japan). Phosphoramidone was obtained from Roche Applied Science. ...


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Caspase Inhibitor ZVAD-fmk Facilitates Engraftment of Donor Hematopoietic Stem Cells in Intra–Bone Marrow–Bone Marrow Transplantation

Caspase Inhibitor ZVAD-fmk Facilitates Engraftment of Donor Hematopoietic Stem Cells in Intra–Bone Marrow–Bone Marrow Transplantation

Y Imai, Y Adachi, M Shi, C Shima… - Stem cells and …, 2010 - online.liebertpub.com
... CA). ZVAD-fmk, an inhibitor of caspases, was purchased from Peptide Institute, Inc.
(Minoh, Japan). PerCP-Cy5.5-labeled avidin was obtained from BD Pharmingen.
Viaprob (BD Pharmingen) was used to eliminate dead cells. ...


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Photocontrol of biological activities of protein by means of a hydrogel

Photocontrol of biological activities of protein by means of a hydrogel

S Murayama, M Kato - Analytical chemistry, 2010 - ACS Publications
... Osaka, Japan). 1-Hydroxybenzotriazole (HOBt), 2-(1H-benzotriazole-a-yl)-1,1,3,3,-
tetramethyluronium hexafluorophosphate (HBTU), and thermolysin were purchased
from Peptide Institute (Osaka, Japan). Elastase pancreatic ...


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Functional analysis of the cucumisin propeptide as a potent inhibitor of its mature enzyme

Functional analysis of the cucumisin propeptide as a potent inhibitor of its mature enzyme

M Nakagawa, M Ueyama, H Tsuruta, T Uno… - Journal of Biological …, 2010 - ASBMB
... Reagents. Restriction and modification enzymes were obtained from New England Biolabs, Roche
Applied Science, and Promega Corp. Glutaryl-l-alanyl-l-alanyl-l-prolyl-l-leucine p-nitroanilide
(Glt-Ala-Ala-Pro-Leu-pNA) was purchased from Peptide Institute (Osaka, Japan). ...


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Expression and activity of N-myristoyltransferase in lung inflammation of cattle and its role in neutrophil apoptosis

Expression and activity of N-myristoyltransferase in lung inflammation of cattle and its role in neutrophil apoptosis

A Shrivastav, SS Suri, R Mohr, KS Janardhan… - Veterinary …, 2010 - vetres.org
... Chemiluminescence reagent plus was obtained from NEN Life Science products (Boston,
USA). Peptide substrate based on the N-terminal ends of pp60 c-Src (GSSKSKPKR) was
synthesized by the Alberta Peptide Institute, Edmonton, Canada. ...


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MEK–ERK-dependent multiple caspase activation by mitochondrial proapoptotic Bcl-2 family proteins is essential for heavy ion irradiation-induced glioma cell …

MEK–ERK-dependent multiple caspase activation by mitochondrial proapoptotic Bcl-2 family proteins is essential for heavy ion irradiation-induced glioma cell …

A Tomiyama, K Tachibana, K Suzuki, S Seino… - Cell death & …, 2010 - ncbi.nlm.nih.gov
... Anti-Fas antibody and anti-FADD antibody were purchased from BD Transduction Laboratories
(Mississauga, ON, Canada). z-VAD-FMK, Ac-DEVD-CHO, AC-LEHD-CHO, and
AC-IETD-CHO were purchased from Peptide Institute Inc. (Osaka, Japan). ...


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IL-1 plays an important role in the bone metabolism under physiological conditions

IL-1 plays an important role in the bone metabolism under physiological conditions

YM Lee, N Fujikado, H Manaka, H Yasuda… - International …, 2010 - Jpn Soc Immunol
... (Tokyo, Japan). Recombinant mouse IL-1Ra was obtained from R&D Systems. Human PTH
(1–34) was obtained from the Peptide Institute (Osaka, Japan). Histology. After killing the mice
by cervical dislocation, femurs were dissected free of surrounding tissues. ...


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1, 2-Dilinoleoyl-sn-glycero-3-phosphoethanolamine ameliorates age-related spatial memory deterioration by preventing neuronal cell death

1, 2-Dilinoleoyl-sn-glycero-3-phosphoethanolamine ameliorates age-related spatial memory deterioration by preventing neuronal cell death

T Yaguchi, T Nagata… - Behavioral …, 2010 - behavioralandbrainfunctions. …
... PC-12 cells were treated with amyloid-β 1-40 peptide (Peptide Institute Inc., Osaka, Japan) or
thapsigargin (Wako, Osaka, Japan) in the presence and absence of phospholipids such as
DLPhtEtn (Avanti Polar Lipid, Inc., Alabaster, AL, USA), 1-linoleoyl-2-palmitoyl-sn-glycero-3 ...


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The mechanism of LPS-induced HIV type I activation in transgenic mouse macrophages

The mechanism of LPS-induced HIV type I activation in transgenic mouse macrophages

M Kadoki, BI Choi, Y Iwakura - International immunology, 2010 - Jpn Soc Immunol
... ERK inhibitor U-0126 (28) and JNK inhibitor SP600125 (29) were obtained from
Calbiochem (La Jolla, CA, USA). Escherichia coli-type synthetic lipid A (compound
506) was purchased from the PEPTIDE Institute (Osaka, Japan). ...


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Terahertz time‐domain spectroscopy of poly‐L‐lysine

Terahertz time‐domain spectroscopy of poly‐L‐lysine

O Kambara, A Tamura, T Uchino, K Yamamoto… - …, 2010 - Wiley Online Library
... All the samples are commercially available powders: poly-L-lysine (Peptide Institute), polyglycine
(Sigma, St. Louis), poly-L-alanine (Miles-Yeda, Rehovot, Israel), glycine, L-alanine, and L-lysine
monohydrochloride (Sigma). The samples were used without further purification. ...


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Changes in the distribution of corticotropin-releasing factor (CRF)-like immunoreactivity in the larval bullfrog brain and the involvement of CRF in the cessation of food …

Changes in the distribution of corticotropin-releasing factor (CRF)-like immunoreactivity in the larval bullfrog brain and the involvement of CRF in the cessation of food …

K Matsuda, N Morimoto, K Hashimoto, R Okada… - General and …, 2010 - Elsevier
... Specificity of the immunostaining was checked by incubating the sections with normal rabbit serum
instead of the primary antiserum, or with 1 ml of anti-CRF serum (diluted 1:4000) preincubated
with excess (20 μM) synthetic ovine CRF (Peptide Institute, Inc., Osaka, Japan). ...


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Regulation of STIM1 and SOCE by the ubiquitin-proteasome system (UPS)

Regulation of STIM1 and SOCE by the ubiquitin-proteasome system (UPS)

JM Keil, Z Shen, SP Briggs, GN Patrick - PLoS One, 2010 - journals.plos.org
The ubiquitin proteasome system (UPS) mediates the majority of protein degradation in eukaryotic
cells. The UPS has recently emerged as a key degradation pathway involved in synapse
development and function. In order to better understand the function of the UPS at synapses ...


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Endostatin expression in neurons during the early stage of cerebral ischemia is associated with neuronal apoptotic cell death in adult hypertensive rat model of stroke

Endostatin expression in neurons during the early stage of cerebral ischemia is associated with neuronal apoptotic cell death in adult hypertensive rat model of stroke

Q Hou, L Ling, F Wang, S Xing, Z Pei, J Zeng - Brain research, 2010 - Elsevier
... The fluorescent assay for CB activity was based on that of Yamashima et al. (1998) adapted for
use in a 96-well plate. Z-Arg-Arg-7-amido-4-methylcoumarin (AMC) (Peptide Institute Inc.,) was
used as substrate, and free AMC (Calbiochem) was used as standard control. ...


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Role of His16 in the structural flexibility of the C-terminal region of human endothelin-1

Role of His16 in the structural flexibility of the C-terminal region of human endothelin-1

H Hiramatsu, H Aduma, Y Tanaka, T Miura… - Journal of Molecular …, 2010 - Elsevier
... other residues. 2. Experimental. 2.1. Materials. HPLC-purified WT ET-1 and its H16A
mutant (acetic acid salt, >95% pure) were purchased from Peptide Institute Inc. (Osaka,
Japan) and used without further purification. The concentration ...


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A Notch ligand, Delta-like 1 functions as an adhesion molecule for mast cells

A Notch ligand, Delta-like 1 functions as an adhesion molecule for mast cells

A Murata, K Okuyama, S Sakano, M Kajiki… - The Journal of …, 2010 - Am Assoc Immnol
... Sodium azide and DMSO were purchased from Wako Pure Chemical Industries (Osaka, Japan).
A γ-secretase inhibitor, N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT)
was purchased from Peptide Institute (Osaka, Japan). Cell lines. ...


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