Changes in the distribution of corticotropin-releasing factor (CRF)-like immunoreactivity in the larval bullfrog brain and the involvement of CRF in the cessation of food …

Changes in the distribution of corticotropin-releasing factor (CRF)-like immunoreactivity in the larval bullfrog brain and the involvement of CRF in the cessation of food …

K Matsuda, N Morimoto, K Hashimoto, R Okada… - General and …, 2010 - Elsevier

... Specificity of the immunostaining was checked by incubating the sections with normal rabbit serum
instead of the primary antiserum, or with 1 ml of anti-CRF serum (diluted 1:4000) preincubated
with excess (20 μM) synthetic ovine CRF (Peptide Institute, Inc., Osaka, Japan). ...

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