High-level expression and characterization of two serine protease inhibitors from Trichinella spiralis

High-level expression and characterization of two serine protease inhibitors from Trichinella spiralis

Z Zhang, Y Mao, D Li, Y Zhang, W Li, H Jia… - Veterinary …, 2016 - Elsevier

... parameters (K m and K cat ) of the proteases were obtained using a reaction mixture containing
2 nM protease for trypsin or 3 nM protease for α-chymotrypsin and pepsin; 50, 57, 66, 80, 100,
or 130 μM Peptidyl-MCA substrates (MCA substrate) (Peptide Institute, Osaka, Japan ...

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