Regulation of ghrelin signaling by a leptin-induced gene, negative regulatory element-binding protein, in the hypothalamic neurons
T Komori, A Doi, H Furuta, H Wakao, N Nakao… - Journal of Biological …, 2010 - ASBMB
... At 3 days after the transfection of mock or NREBP, GT1-7 cells were treated with saline or ghrelin
(Peptide Institute, Osaka, Japan) dissolved with saline at a dose of 100 nm. Five min after treatment,
the cells were used as samples for Western blot analysis. Western Blot Analysis. ...
(Peptide Institute, Osaka, Japan) dissolved with saline at a dose of 100 nm. Five min after treatment,
the cells were used as samples for Western blot analysis. Western Blot Analysis. ...
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