Site‐specific cleavage of acetoacetyl‐CoA synthetase by legumain

Site‐specific cleavage of acetoacetyl‐CoA synthetase by legumain

S Hasegawa, D Inoue, M Yamasaki, C Li, M Imai… - FEBS …, 2016 - Wiley Online Library

... buffer (40 mm citric acid, 121 mm Na 2 HPO 4 , pH5.8, containing 1 mm dithiothreitol, 1 mm EDTA,
and 0.1% CHAPS) including 10 μm AEP substrate [benzyloxycarbonyl-l-alanyl-l-alanyl-l-
asparagine-4-methylcoumaryl-7-amide (Z-Ala-Ala-Asn-MCA; Peptide institute, INC., Osaka ...

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