Subcutaneous allergic sensitization to protease allergen is dependent on mast cells but not IL-33: distinct mechanisms between subcutaneous and intranasal routes

Subcutaneous allergic sensitization to protease allergen is dependent on mast cells but not IL-33: distinct mechanisms between subcutaneous and intranasal routes

S Kamijo, M Suzuki, M Hara, S Shimura… - The Journal of …, 2016 - Am Assoc Immnol

... To prepare papain treated with E64 (Peptide Institute, Osaka, Japan), similar to our previous
studies (10, 16), papain was reacted with an excess of E64 after the addition of L-cysteine and,
after the reaction, L-cysteine and unbound free E64 were removed by dialysis (E64 ...

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