A novel Leishmania major amastigote assay in 96-well format for rapid drug screening and its use for discovery and evaluation of a new class of leishmanicidal …

A novel Leishmania major amastigote assay in 96-well format for rapid drug screening and its use for discovery and evaluation of a new class of leishmanicidal …

G Bringmann, K Thomale, S Bischof… - Antimicrobial agents …, 2013 - Am Soc Microbiol

... A volume of 5 μl protein lysate was mixed with 89 μl acidic sodium acetate buffer for each sample,
followed by addition of 1 μl DMSO, 1 μl 10 mM epoxysuccinyl peptide l-trans-epoxysuccinyl-Leu-
4-guanidinobutylamide (E64; Bachem), or 1 μl 5 mM l-trans-epoxysuccinyl-Ile-Pro ...

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