A single mutation within a Ca 2+ binding loop increases proteolytic activity, thermal stability, and surfactant stability

A single mutation within a Ca 2+ binding loop increases proteolytic activity, thermal stability, and surfactant stability

M Okuda, T Ozawa, M Tohata, T Sato, K Saeki… - … et Biophysica Acta (BBA …, 2013 - Elsevier

... nitroanilide (AAPL). All synthetic peptides were obtained from Bachem except for
AAPF (Sigma) and AAPL (Peptide Institute). Peptidase assays were performed at
30 °C using peptides in 50 mM borate buffer (pH 10). Released ...

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