The ATP costs and time required to degrade ubiquitinated proteins by the 26 S proteasome

The ATP costs and time required to degrade ubiquitinated proteins by the 26 S proteasome

A Peth, JA Nathan, AL Goldberg - Journal of Biological Chemistry, 2013 - ASBMB

... Hydrolysis of the small peptide succinyl-GGL-7-amino-4-methyl coumarin (Bachem) by
proteasomes ... radiolabeling it with protein kinase A and [ 32 P]ATP (22) and then following the
conversion of the 32 P-radiolabeled substrate to acid-soluble 32 P-labeled peptides after TCA ...

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