The molecular cell death machinery in the simple cnidarian Hydra includes an expanded caspase family and pro-and anti-apoptotic Bcl-2 proteins
M Lasi, B Pauly, N Schmidt, M Cikala, B Stiening… - Cell …, 2010 - search.proquest.com
... for 2 h at room temperature in assay buffer (25 mM HEPES (pH 7.5), 0.1 % (w/v) CHAPS, 10 mM
DTT, 10 g/ml pepstatin A, 10 g/ml aprotinin, 10 g/ml leupeptin, 10 mM pefabloc) with 1 M of the
active-site labeling reagents z-EK(biotin)D-AOMK (Peptide Institute Inc, Japan) or FITC ...
DTT, 10 g/ml pepstatin A, 10 g/ml aprotinin, 10 g/ml leupeptin, 10 mM pefabloc) with 1 M of the
active-site labeling reagents z-EK(biotin)D-AOMK (Peptide Institute Inc, Japan) or FITC ...
没有评论:
发表评论