Directed Evolution Empowered Redesign of Natural Proteins for the Sustainable Production of Chemicals and Pharmaceuticals

Directed Evolution Empowered Redesign of Natural Proteins for the Sustainable Production of Chemicals and Pharmaceuticals

UT Bornscheuer, B Hauer, KE Jaeger… - Angewandte Chemie …, 2019 - Wiley Online Library

… After expression of the phage DNA ⑦, the target peptide is displayed as a fusion with the host
capsule protein … In a single round of affinity purification, Smith succeeded in enriching the phages
presenting peptide fragments by a factor of 1000 over other phages …

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