Inhibition of neural activity depletes orexin from rat hypothalamic slice culture

Inhibition of neural activity depletes orexin from rat hypothalamic slice culture

S Michinaga, A Hisatsune, Y Isohama… - Journal of …, 2010 - Wiley Online Library

... NMDA and amlodipine besylate were from Wako Chemicals (Osaka, Japan). ω-Conotoxin MVIIC
was from the Peptide Institute (Osaka, Japan). At 18 days in vitro, drug treatment was achieved
by transferring culture inserts into wells containing culture medium with added drugs. ...

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