Molecular basis of photochromism of a fluorescent protein revealed by direct 13C detection under laser illumination
... Bacteria were disrupted by repeated freezing/thawing in the presence of lysozyme (250 μg/ml,
Sigma–Aldrich), DNaseI (250 μg/ml, Sigma–Aldrich), 1 mM MgCl 2 , and proteinase inhibitors
(10 μM E-64, 10 μM leupeptin, 1 μM pepstatin A, Peptide Institute; 50 μM ...
Sigma–Aldrich), DNaseI (250 μg/ml, Sigma–Aldrich), 1 mM MgCl 2 , and proteinase inhibitors
(10 μM E-64, 10 μM leupeptin, 1 μM pepstatin A, Peptide Institute; 50 μM ...
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