2017年5月31日星期三

Activation of caspase-9, but not caspase-2 or caspase-8, is essential for heat-induced apoptosis in Jurkat cells

Activation of caspase-9, but not caspase-2 or caspase-8, is essential for heat-induced apoptosis in Jurkat cells

SN Shelton, CD Dillard, JD Robertson - Journal of Biological Chemistry, 2010 - ASBMB
... Cells were resuspended in 25 μl of PBS, added to a microtiter plate, and combined with
DEVD-aminomethylcoumarin (Peptide Institute, Osaka, Japan) dissolved in a standard reaction
buffer (100 mm Hepes, pH 7.25, 10% sucrose, 10 mm dithiothreitol, 0.1% CHAPS). ...


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