2017年5月31日星期三

Assessment of Homologous Internalization of Constitutively Active N111G Mutant of AT1 Receptor

Assessment of Homologous Internalization of Constitutively Active N111G Mutant of AT1 Receptor

MA Bhuiyan, T Nagatomo - Methods in enzymology, 2010 - books.google.com
... Briefly, for saturation binding studies, six to seven concentrations (5–800 pM) of
[125I]-Sar1-Ile8-Ang II were tested in duplicate. Nonspecific binding was defined as the amount
of radioligand binding remaining in presence of 10 mM Ang II (Peptide Institute Inc. Japan). ...


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