Expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets
E Heimann, HA Jones, S Resjö, VC Manganiello… - PloS one, 2010 - journals.plos.org
... Cells were washed three times with chilled homogenization buffer containing 250 mM sucrose,
0.5 mM EGTA, 5 mM HEPES adjusted with KOH to pH 7.4, supplemented with 10 µg/ml leupeptin,
10 µg/ml antipain and 1 µg/ml pepstatin (Peptide Institute Inc., Osaka, Japan), 5 mM ...
0.5 mM EGTA, 5 mM HEPES adjusted with KOH to pH 7.4, supplemented with 10 µg/ml leupeptin,
10 µg/ml antipain and 1 µg/ml pepstatin (Peptide Institute Inc., Osaka, Japan), 5 mM ...
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