Functional hypothalamic amenorrhea due to increased CRH tone in melanocortin receptor 2-deficient mice
T Matsuwaki, M Nishihara, T Sato, T Yoda… - …, 2010 - press.endocrine.org
... an sc injection of estradiol benzoate (1 μg in 0.1 ml sesame oil, Sigma-Aldrich) (n 7 mice per
genotype per treatment) at 0800 h. At 0800 h of the morning after the hormone/ vehicle injection,
mice received an ip injection of either GnRH (400 ng/kg in 0.1% PBS; Peptide Institute. ...
genotype per treatment) at 0800 h. At 0800 h of the morning after the hormone/ vehicle injection,
mice received an ip injection of either GnRH (400 ng/kg in 0.1% PBS; Peptide Institute. ...

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