Isolation of Inositol 1, 4, 5-Trisphosphate Receptor-Associating Proteins and Selective Knockdown Using RNA Interference
A Mizutani, K Kawaai, C Hisatsune, H Ando… - Inositol Phosphates and …, 2010 - Springer
... 4. Sf 9 cell homogenization buffer: 10 mM HEPES (pH 7.4), 100 mM NaCl, 2 mM EDTA, 1 mM
2-mercaptoethanol, 0.1% Triton X-100, and a cocktail of protease inhibitors: 1 mM
phenylmethylsulfonyl fluoride (Sigma-Aldrich), 10 µM leupeptin (Peptide Institute), 2 µM pepstatin ...
2-mercaptoethanol, 0.1% Triton X-100, and a cocktail of protease inhibitors: 1 mM
phenylmethylsulfonyl fluoride (Sigma-Aldrich), 10 µM leupeptin (Peptide Institute), 2 µM pepstatin ...
Peptide synthesis, peptide modification and peptide library synthesis, please contact:
Eva He
eva@pepmic.com
Pepmic Co., Ltd
www.pepmic.com
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