PAC1 gene knockout reveals an essential role of chaperone-mediated 20S proteasome biogenesis and latent 20S proteasomes in cellular homeostasis

PAC1 gene knockout reveals an essential role of chaperone-mediated 20S proteasome biogenesis and latent 20S proteasomes in cellular homeostasis

K Sasaki, J Hamazaki, M Koike, Y Hirano… - … and cellular biology, 2010 - Am Soc Microbiol

... Assay of proteasome activity.The peptidase activity of the proteasome was measured using
a fluorescent peptide substrate, succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
(Suc-LLVY-MCA; Peptide Institute), as described previously (55). ...

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