A GPI signal peptide-anchored split-ubiquitin (GPS) system for detecting soluble bait protein interactions at the membrane
14 Plant Physiol. Vol. 178, 2018 peptide sequence (Fig. 1B) in front of the Gateway cassette
of the bait vector pMetOYC-Dest of the CytoSUS system (Karnik et al., 2015) using unique
restriction sites XhoI and XbaI (GenScript USA; HindIII also was introduced for future cloning …
of the bait vector pMetOYC-Dest of the CytoSUS system (Karnik et al., 2015) using unique
restriction sites XhoI and XbaI (GenScript USA; HindIII also was introduced for future cloning …
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