2018年11月12日星期一

Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

Z Shimatani, S Kashojiya, M Takayama, R Terada… - Nature …, 2017 - nature.com
… were introduced by PCR and Gibson assembly methods (Supplementary Table 6). The
Arabidopsis codon– optimized coding sequence of PmCDA1 was synthesized by Eurofins
Genomics (Tokyo, Japan) and inserted after d/nCas9 with the same linker peptide, as described …


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