Signal peptide-dependent protein translocation pathway is crucial for the sucrose sensitivity of SacB-expressing Escherichia coli

Signal peptide-dependent protein translocation pathway is crucial for the sucrose sensitivity of SacB-expressing Escherichia coli

S Gao, S Yao, DJ Hart, Y An - Biochemical Engineering Journal, 2017 - Elsevier

Escherichia coli expressing the levansucrase SacB gene cannot grow on LB plates
containing 5% sucrose, therefore this gene has been used as a counter-selection marker in
many studies. However, the mechanism of this phenomenon is still unclear. In this study, we …

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