Human mtGPD

Product	Human mtGPD
Cat#	101-5-07A
Sequence	His-Tag sequence not included: ADCISEPVNREPPSREAQLLTLQNTSEFDILVIGGGATGSGCALDAVTRGLKTALVERDD FSSGTSSRSTKLIHGGVRYLQKAIMKLDIEQYRMVKEALHERANLLEIAPHLSAPLPIML PVYKWWQLPYYWVGIKLYDLVAGSNCLKSSYVLSKSRALEHFPMLQKDKLVGAIVYYDGQ HNDARMNLAIALTAARYGAATANYMEVVSLLKKTDPQTGKVHVSGARCKDVLTGQEFDVR AKCVINATGPFTDSVRKMDDKDAAAICQPSAGVHIVMPGYYSPESMGLLDPATSDGRVIF FLPWQKMTIAGTTDTPTDVTHHPIPSEEDINFILNEVRNYLSCDVEVRRGDVLAAWSGIR PLVTDPKSADTQSISRNHVVDISESGLITIAGGKWTTYRSMAEDTINAAVKTHNLKAGPS RTVGLFLQGGKDWSPTLYIRLVQDYGLESEVAQHLAATYGDKAFEVAKMASVTGKRWPIV GVRLVSEFPYIEAEVKYGIKEYACTAVDMISRRTRLAFLNVQAAEEALPRIVELMGRELN WDDYKKQEQLETARKFLY
Unit/Weight	0.01 mg
Unit Price	$228.00
Description	Mitochondrial glycerol-3-phosphate dehydrogenase (mtGPD) is a Ca2+-sensitive, FAD-binding protein located on the outer surface of the inner mitochondrial membrane. mtGPD catalyses the oxidation of glycerol-3-phosphate to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Two isoforms have been described for mtGPD. Isoform 1 comprises 727 aa residues, whereas isoform 2 lacks 126 aa residues of the N-terminus. Deficiency of mGPD appears to contribute to the impairment of glucose-stimulated insulin release in several animal models of non-insulin dependent diabetes mellitus. Recombinant mtGPD comprises a 558 amino acid fragment (43-600) corresponding to the GlpA domain fragment of the mature mtGPD protein and is expressed in E. coli with an amino-terminal hexahistidine tag.
Molecular Weight	66.26 kDa
Purity	Purity > 95% as determined by reducing and non-reducing SDS-PAGE, Bradford assay, Western blot and ELISA
Storage	Store at -20°C. Product is guaranteed one year from the date of shipment.