Hydrophilic strong anion exchange (hSAX) chromatography for highly orthogonal peptide separation of complex proteomes

Hydrophilic strong anion exchange (hSAX) chromatography for highly orthogonal peptide separation of complex proteomes

MS Ritorto, K Cook, K Tyagi… - Journal of proteome …, 2013 - ACS Publications

Due to its compatibility and orthogonality to reversed phase (RP) liquid chromatography
(LC) separation, ion exchange chromatography, and mainly strong cation exchange (SCX),
has often been the first choice in multidimensional LC experiments in proteomics. Here, we …

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